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to investigate the constraints imposed by Lon within the DHFR mutational landscape in structural detail, we defined a ∆assortment coefficient for every amino acid residue at Each individual posture as the distinction between the +Lon and –Lon selections (determine 3A). The ∆choice coefficient values ended up most adverse at positions within the advantageous classification and at positions with a native VILMWF or Y amino acid residue (Figure 3B, excludes Intolerant positions from –Lon range); General, mutations at positions with native hydrophobic residues are enriched for destructive ∆variety coefficients (Figure 3—figure supplement 1A).
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